5-Amino-1MQ works by inhibiting an enzyme called nicotinamide N-methyltransferase (NNMT). This enzyme plays a key role in metabolic regulation and energy balance. By reducing NNMT activity, 5-Amino-1MQ influences fat metabolism and cellular energy processes.
Its primary mechanism centers on enhancing nicotinamide adenine dinucleotide (NAD+) levels, an essential molecule for numerous biological processes, including mitochondrial function and cellular repair.
The inhibition of NNMT has broader implications for obesity-related issues, muscle wasting, and age-related metabolic decline. Its function at the molecular level makes it a promising therapeutic agent in addressing these conditions.
 Weight loss and fat metabolism
Promotes fat oxidation: Inhibiting NNMT activity is suggested to accelerate fat oxidation, potentially aiding in fat loss, especially when combined with exercise and diet.
Reduced fat storage and adipocyte size: Studies on mice showed reduced body weight, white adipose (fat) mass, and adipocyte (fat cell) size in those treated with 5-Amino-1MQ, without affecting food intake.
Enhanced metabolism: By blocking NNMT, 5-Amino-1MQ may boost cellular energy expenditure and fat burning by increasing NAD+ levels (a vital coenzyme in metabolism). 
 Muscle preservation and function
Prevents muscle wasting: By enhancing energy production and improving mitochondrial efficiency, 5-Amino-1MQ may help maintain lean muscle mass, especially important for aging individuals or those with certain medical conditions.
Improved muscle function and strength: Research in aged mice showed 5-Amino-1MQ treatment increased grip strength, and when combined with rigorous exercise, further enhanced grip strength and allowed for longer running distances compared to exercise alone.
Potential for muscle repair and rejuvenation: Studies suggest 5-Amino-1MQ may help rejuvenate senescent muscle stem cells, enhancing their ability to repair and replace damaged muscle fibers, potentially benefiting age-related muscle loss and conditions like muscular dystrophy. 
 Improved energy levels and anti-aging potential
Enhanced energy production: The increase in NAD+ levels supported by 5-Amino-1MQ directly impacts cellular energy production, leading to reports of improved physical and mental energy.
Anti-aging properties: By regulating metabolic processes, improving cellular energy production, and promoting fat burning, 5-Amino-1MQ may help reduce the visible signs of aging related to metabolic slowdown. 
 Other potential benefits
Improved metabolic health: 5-Amino-1MQ may help regulate the body's metabolic pathways, potentially leading to improved insulin sensitivity and better blood sugar control, which can be beneficial for those looking to optimize metabolic health as they age.